Organisation
Preliminary talk
Before sorting of samples the aims and conditions have to be clarified with the operator. In case of standard sort procedures (human or mouse samples, standard dyes) this can be done via E-mail/telephone. The estimate sorting time and adjust the sorter set-up, the operator needs information on cell type (size, origin, infectious material), cell numbers (total, target cells), used fluorochromes and subsequent cell use (in vitro cultures, mRNA isolation etc.).